Excision of a microinjected Tol2-based construct.

Abstract

<p>(<b>A</b>) Scheme of donor vector pTol2{rps9::egfp}^frkt868; the rps::egfp cassette is flanked by inverted Tol2 repeats (Tol2IR) that serve as recognition site for Tol2 transposase co-injected as mRNA along with the vector DNA. (<b>B, C</b>) PCR using vector-specific primers (black arrows) yields a 200 bp PCR fragment specifically from embryos co-injected with <i>tol2</i> transposase mRNA (left lane), but not from controls (right lane). (<b>D</b>) Precise cleavage of the reporter construct at the end of the Tol2 IRs is evidenced by sequencing of the 200 bp fragment.</p