Effect of free DOX and DOX- PCL63-b-PNVP90 on normal Cell viability.
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Abstract
<p>Normal human lymphocytes (A), DC (B), and monocytes (C) were treated with serial concentrations of doxorubicin (0.0001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, and 5 μM). Plates were incubated at 37°C, 5% CO<sub>2</sub>, for 48 h. The cell viability was measured by XTT assay kit (Cell Signaling, USA). Data shown as mean ± SD, n = 3. Effects on leukocytes number following in vivo administration of free DOX and DOX-PCL<sub>63</sub>-b-PNVP<sub>90</sub> was studied in normal mice (D) tumor bearing mice (E) mice treated with free DOX (F), or DOX- PCL<sub>63</sub>-b-PNVP<sub>90</sub> (G). RBC (H) and differential leukocyte count (I) of individual treatment are shown, mean ± SD, n = 3. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 in control versus experimental group).</p
