<p>(<b>A</b>) Genomic Southern blot with an <i>egfp</i> probe reveals genomic integrations of pTol2{rps9::egfp}<sup>frkt868</sup> in tested G<sub>0</sub> individuals (arrows). (<b>B</b>) Precise genomic integration of a the reporter construct at the end of the IR as revealed by sequencing of an amplicon including neighboring genomic DNA, generated using Tol2IR-specific TAIL-PCR primers. (<b>C</b>) Integration and inheritance of <i>egfp</i> in individual G<sub>0</sub> and G<sub>1</sub> animals: PCR amplicons specific for <i>egfp</i> (arrowheads) can be detected in genomic DNA of both G<sub>0</sub> (lanes 9–18) and G<sub>1</sub> (lanes 1–8) individuals. Controls: no DNA (lane 19), wild-type gDNA (lane 20), gDNA extracted from a tuba::egfp<sup>vbci1</sup> transgenic individual (lane 21), reporter construct plasmid DNA (lane 22).</p
