Induction of extracellular viral RNA release from CD4 T cells treated with RMD and VOR.

Abstract

<p>Memory or resting CD4 T cells isolated from HIV-infected patients on suppressive cART were treated with RMD or VOR, and viral RNA was quantified in cell culture supernatants 6 days after the addition of drugs. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004071#s2" target="_blank">Results</a> are depicted as fold increase in viral RNA relative to control cultures. Each symbol represents one HIV subject. Solid circles, p<0.05; open circles, p>0.05 compared to vehicle-treated controls from the same donors; solid squares, p value not calculated. Red lines represent the mean fold HIV induction across all analyzed donors. Symbols # and * denote a statistically significant difference (p<0.05) for RMD-mediated HIV induction vs. 0.5 and 1 µM VOR, respectively, across all tested donors. Data for each individual donor and condition including results of the statistical analysis are summarized in Supplementary <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004071#ppat.1004071.s008" target="_blank">Table S4</a>. (<b>A</b>) Memory CD4 T cells were treated with RMD and VOR for 4 and 24 hours, respectively. (<b>B</b>) Memory CD4 T cells were treated continuously for 6 days. (<b>C</b>) Resting CD4 T cells were treated continuously for 6 days.</p