Activation of heterochromatic <i>hsp82-2001</i> occurs in the presence of minimal H2A.Z levels and is unimpaired by deletion of <i>HTZ1</i>.

Abstract

<p>(a) ChIP analysis of H2A.Z at the indicated <i>hsp82</i> alleles under NHS (−) and 20 min HS (+) conditions conducted as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004202#pgen-1004202-g004" target="_blank">Figure 4B</a>, with H2A.Z content normalized per nucleosome (Myc-H4 abundance). Shown are means ± S.D. (N = 2). (b) <i>hsp82-2001</i> transcript abundance was determined in WT and <i>htz1Δ</i> cells (<i>sir4Δ</i> and <i>SIR⁺</i> as indicated) subjected to heat shock for the indicated periods of time. RNA was isolated, cDNA synthesized and <i>HSP82</i> transcripts were quantified using RT-qPCR and normalized to <i>SCR1</i> as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004202#pgen-1004202-g004" target="_blank">Figure 4A</a>. Depicted are means ± S.D. (N = 2; qPCR = 4).</p