<p>(a) ChIP-qPCR analysis of Pol II occupancy at the promoter, ORF and 3′-UTR of the <i>hsp82-2001</i> gene in <i>sir4Δ</i> and <i>SIR<sup>+</sup></i> cells heat shocked for the indicated times. All values represent net ChIP signals (immune – pre-immune serum) at <i>hsp82-2001</i> normalized to those at <i>ARS504</i>. Pol II occupancy at the euchromatic <i>hsp82-2001</i> promoter was set to 1.0; all other occupancies are scaled relative to this. (b) ChIP analysis of Cet1 occupancy of <i>hsp82-201</i>, <i>hsp82-1001</i> and <i>hsp82-2001</i> under NHS (−) and 20 min HS (+) conditions, conducted as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004202#pgen-1004202-g002" target="_blank">Figure 2A</a>. Left, gel analysis. Input sample (lane 1) represents 0.4% of soluble chromatin used in each IP. Mock, chromatin reacted with beads alone. Lanes 3–10, chromatin isolated from <i>SIR<sup>+</sup></i> and <i>sir4Δ</i> strains as indicated (+ and −, respectively) and immunoprecipitated with a Cet1-specific antibody. Histogram represents net mean values (immune signal minus beads alone) of three independent experiments.</p
