Activation of heterochromatic genes occurs in the context of negligible H4K12 acetylation.

Abstract

<p>(a) H4K12ac enrichment at <i>hsp82-2001</i> subjected to heat shock for the indicated times. H4K12Ac and H3 ChIP signals were quantified at promoter, ORF and 3′-UTR using Real Time qPCR and normalized to those measured at <i>PMA1</i> and <i>ARS504</i> (for H4K12ac and H3, respectively). H4K12ac/H3 quotients were then determined and scaled to the non-induced promoter signal in <i>sir4Δ</i> cells, which was set to 1. Depicted are means ± S.D (N = 2; qPCR = 4). (b) H4K12ac enrichment at <i>YFR057W</i> promoter and ORF at the indicated times following addition of 200 µg/ml cycloheximide. Quantification was performed as in A.</p