<div><p>A new ceramide, 2,3-dihydroxy-<i>N</i>-[(2<i>S</i>,3<i>S</i>,4<i>R</i>)-1,3,4-trihydroxyicosan-2-yl[tetracosanamide (<b>1</b>) along with four known compounds: 2-(4′-hydroxyphenyl)-ethyl lignocerate (<b>2</b>), docosyl-3,4-dihydroxy-<i>trans</i>-cinnamate (<b>3</b>), β-sitosterol (<b>4</b>) and β-sitosterol glycoside (<b>5</b>) were isolated from <i>Tinospora oblongifolia</i> (Menispermaceae). Their structures were determined on the basis of spectroscopic methods, mass spectrometry analysis as well as chemical transformation and by comparing their physical and spectral data with those reported in the literature. Compound <b>1</b> exhibited strong cytotoxic activity against KB cells with IC<sub>50</sub> = 3.4 μM although less than that of camptothecin IC<sub>50</sub> = 0.3 μM (positive control).</p></div
