<p>(A) SH-SY5Y cells were treated for 48 h with 5 µM 7β-hydroxycholesterol (7β-OH), 24-hydroxycholesterol (24-OH), 27-hydroxycholesterol (27-OH) or 15 µM oxysterol mixture (Mix). Untreated cells (Control) were taken as controls. COX-2 levels were analyzed by Western blotting. Top: blot representative of three experiments. Bottom: histogram representing mean values ± SD of three experiments. COX-2 densitometric measurements were normalized against the corresponding actin levels and expressed as percentages of control value.**P<0.01 and ***P<0.001 vs. control. (B) mPGES-1 expression was quantified by real-time RT-PCR in SH-SY5Y cells treated for 6 h with 5 µM 7β-OH, 24-OH, 27-OH or 15 µM oxysterol mixture. Some cells were pretreated for 1 h with 5 µM free quercetin (Q<sub>F</sub>) or with 5 µM quercetin loaded into nanoparticles (Q<sub>N</sub>) before oxysterol treatment. Untreated cells (Control) were taken as controls. Data, normalized to β<sub>2</sub>-microglobulin, are expressed as mean values ± SD of three different experiments. ***P<0.001 vs. control; ###P<0.001 vs. specific oxysterol.</p
