TIPE2-deficient deficient mice exhibit greater iNOS induction and NO production in response to LPS challenge compared to WT controls.

Abstract

<p>WT and TIPE2^−/− mice injected intraperitoneal with phosphate buffered saline (PBS) or with LPS (1.5 mg/kg body weight) and sacrificed 3 or 24 h later. Sera concentration of NO and urea were examined (A and B). Liver and lung tissues of these animals were collected to extract total RNA and protein. The mRNA levels of iNOS, arginase I and arginase II in livers (B, D and E, left panels) and lungs (B, D and E, right panels) were examined by quantitative PCR at 3 h post-PBS or LPS challenge. iNOS protein levels in the livers (C, left panel) and lungs (C, right panel) were examined by Western blot at 24 h post-LPS challenge. Data are shown as means ±SE (n = 4) of one representative experiment. *P<0.05; **P<0.01; ***P<0.001.</p