<p>(A) Stimulatory activities of calf serum, lipoprotein-depleted serum, lipoprotein- and albumin-depleted serum were examined. Fractionation was performed from a 50-ml volume of calf serum containing 3850 units of stimulatory activity. Each fraction was applied to soft agar by 2-fold serial dilution and their colony-spreading stimulatory activities on the MRSA NI-15 strain were measured. Relative total activities of each fraction compared with that of calf serum are presented in graph. (B) SDS-PAGE analysis of the lipoprotein-depleted serum and Affi-Gel blue gel column effluent. 10 µg protein of the lipoprotein-depleted serum and the Affi-Gel blue gel column effluent was electrophoresed in 12.5% SDS-polyacrylamide gel.</p
