<p>(A) Colony-spreading stimulation by purified bovine serum albumin. Purified bovine serum albumin of 96% grade (open triangles, Nacalai, cat. no. 01861-26) or 99% grade (open circles, Sigma, cat. no. A0281) was applied to soft agar by 2-fold serial dilution and their colony-spreading stimulatory activities against MRSA NI-15 strain were measured. (B) Elution profile of DEAE-cellulose column chromatography using bovine serum albumin (96% grade, Nacalai). Open circles indicate the colony-spreading stimulatory activity. Filled circles indicate absorbance at 280 nm. (C) SDS-PAGE analysis of DEAE-cellulose column chromatography fractions. The gel was stained with Coomassie Brilliant Blue. The 66-kDa protein coincided with colony-spreading stimulatory activity in fractions 10–20. (D) Bovine serum albumin (Nacalai), fatty acid-free albumin from bovine serum (Wako Chemicals, cat. no. 017-15146), casein from bovine milk (Sigma, cat. no. C4032), or fetuin from calf serum (Sigma, cat. no. F2379) was applied to soft agar by 2-fold serial dilution and their colony-spreading stimulatory activities on the MRSA NI-15 strain were measured.</p
