Establishment of hICD-expressing stable cells.
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Abstract
<p>(A) Transient expression of hTMC construct in HEK293 cells produced, in addition to the expected TMC fragment, a band similar in size to the intracellular domain of FPC. * Ig heavy and light chains. (B) Western blotting showing the levels of hICD in four mIMCD-3 cell lines stably expressing hICD (hICD3, 5, 6, 13), along with four empty vector control cell lines (CTL1, 2, 3, 4). IB, immunoblotting. (C) FPC C-tail was mainly localized in the nuclei of mIMCD-3 cells (hICD3) compared to the control cell line (CTL3). IF, immunofluorescence. Scale bar, 5 µm.</p