Decreased cell proliferation rate and increased apoptosis in hICD-expressing cells.

Abstract

<p>(A) The number of hICD-expressing cells was significantly lower than that of control cells when cultured in 1% but not in 10% serum-containing medium (N = 3 hICD3, 6, 13). One representative of three experiments was presented. (B) With flow cytometry analysis, more hICD-expressing cells passed the restriction point in G1/0-phase but were arrested in S-phase after serum removal for 48 h. Addition of 10% serum for 24 h completely compromised the effect of hICD expression on the cell cycle profile (N = 3 hICD3, 6, 13). One representative of three experiments was presented. (C) More apoptotic hICD-expressing cells were found in the absence of serum by flow cytometry analysis (N = 3 hICD3, 6, 13). One representative of at least three experiments was presented. (D) Three control and three hICD cell lines were used for this experiment. Pictures from two control and hICD cell lines were presented. TUNEL staining assay confirmed an increase of apoptotic hICD-expressing cells. Bar graph represented statistical significance (N = 200 cells from three controls and hICD cell lines). Open bars, control cells; filled bars, hICD-expressing cells. Values were expressed as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001. Scale bar, 10 µm.</p