<p>(<b>A</b>) MAS-PCR: <i>p29</i> genotype profile of the <i>E. granulosus</i> s.s. (G1) by MAS-PCR. Two alleles encoding the P29 protein within <i>E. granulosus</i> s.s. (G1) were identified. Result of a MAS-PCR showed homozygotes A1/A1 (lanes 9 and 10), homozygotes A2/A2 (lanes 1–5) and heterozygotes (lanes 6–8), visualized on a 2% agarose gel. M: 100-bp DNA ladder (Promega). (<b>B</b>) Location of 34 patients used in this study for the <i>p29</i> multiplex allele specific (MAS)-PCR. The main area for human risk is located in Central Tunisia and includes Kairouan, Kasserine and Sidi Bouzid. All isolates were first genotyped as <i>E. granulosus</i> s.s. (G1). Samples identified as homozygote A1/A1 are represented by a black square, homozygotes A2/A2 are signified by a grey triangle and heterozygotes are showed by a white circle with black border.</p
