Competitive Western blot analysis with recEg(G1)P29 expressed from <i>E. granulosus</i> s.s. (G1) and recEcnd(G6) expressed from <i>E. canadensis</i> (G6).
<p>(A) Shows a silver stained gel of expressed and purified recombinant antigens; recEg(G1)P29 (lane 1) and recEcnd(G6)P29 (lane 2). (<b>B</b>) Recombinant P29 proteins were separated by SDS-PAGE under reducing conditions and blotted onto a nitrocellulose membrane. Human serum from a CE patient either infected with <i>E. granulosus</i> s.s. (G1) or <i>E. canadensis</i> (G6) were added to recEg(G1)P29 (strip 1 and 2, respectively) or recEg(G6)P29 (strip 3 and 4, respectively). In a competition assay nitrocellulose strips loaded with recEcnd(G1)P29 were incubated with serum from CE patients either infected with <i>E. granulosus</i> s.s. (G1) or <i>E. canadensis</i> (G6). The sera were pre-incubated with the recombinant expressed competitor recEcnd(G6)P29 (strip 5 and 6) or as a control with recEg14-3-3 (strip 7 and 8). Immune sera were used at dilutions of 1∶100 and competitor/control at concentrations of 1 µg/mL.</p
