Immunohistochemical staining (dark brown coloring) for Bcl-2 protein in lung specimens from chickens incubated under normal and hypoxic conditions.

Abstract

<p>Bcl-2 protein expression in lung samples from nW, hT, and hW gradually increased moderately from E16 to E18 before the onset of lung functioning (E–H), and disappeared at E19. By E18 in nW lungs, weaker Bcl-2 expression was observed relative to hT (G) and hW (H) chicken groups. In nT chicken sections, Bcl-2 protein staining was nearly unchanged throughout the developmental stages examined (A, E, K). To see details of the E18 lung structure, higher-magnification photomicrographs were taken. Bcl-2 staining was detected in the mesenchyme (arrows) around the ACs and not in the infundibula or atrias of chicken lung (F’, G’, H’)”. In the hW section at E18, Bcl-2 staining was strong (arrow in H’), but weaker in hT and nW at this stage (arrows in G’, F’). Scale bar  =  200 µm.</p