HIF-1α and Bcl-2 expression in lung tissue under hypoxia and normoxia conditions.

Abstract

<p>Data were obtained from hT, hW, nT, and nW chicken tissues at E16, E18, E19, and E20. (A) Quantitative expression analysis of HIF-1α mRNA in embryonic lung at E16, E18, E19, and E20. The stress reaction in hW was robust as compared with the smooth response in hT. Data are expressed as the mean ± SEM for each group. (B) Quantitative expression analysis of <i>bcl-2</i> mRNA in the embryonic lung, heart, brain and liver at E16 and E19. There were no changes in the expression of <i>bcl-2</i> mRNA between the E16 and E19 in each kind of embryonic tissues. Data are expressed as the mean ± SEM for each group. (C) Analysis of HIF-1α and Bcl-2 expression at the protein level in lung tissue (shown in western blot and densitometry value). HIF-1α protein expression increased from E16 to E19, however in different level in hT, hW, nT, and nW group. Bcl-2 protein did show different levels of expression across different time points and different groups. (* <i>P</i><0.05; ** P<0.01; ns  =  not significant).</p