The secretion of CXCL16 by lung cancer cell lines <i>in vitro</i>.

Abstract

<p>Digested A549, H292 and 95D cells were seeded in 24-well plates (500 µl/well) at a density of 5×10⁵/ml, respectively. Supernatants of the cell cultures were collected at 24, 36, 48, 60, 72, 96 and 100 h of culture. An ELISA assay was performed to examine the release of the soluble CXCL16 in cultured A549, H292 and 95D cells <i>in vitro</i>. As shown in Fig. 4, three kinds of lung cancer cell lines all secreted CXCL16 spontaneously in a time-dependent manner, despites of a difference in the concentration of CXCL16 in the culture medium. The experiments were repeated three times. Error bars depict the standard error of the mean.</p