Illustration of the “iterative strategy” underlying pyrrolamide biosynthesis.

Abstract

<p>The putative amidohydrolase Pya25 catalyzed the deacetylation of PCP-tethered pyrrolamide biosynthesis intermediates and determined the number of the pyrrole groups assembled into various pyrrolamides. A, adenylation domain; C, condensation domain; PCP, peptidyl carrier protein.</p