Effects of the NF-κB inhibitor and IL-17A on cell invasion and the expressions of MMP-2 and MMP-9 in AGS cells.

Abstract

<p>(A) 1×10⁶ AGS cells were pretreated with helenalin (5 µM) for 30 min and then incubated in the presence or absence of IL-17A (50 ng/ml) for 24 h. Cellular invasiveness was measured using the transwell invasion assay. (B) The percent invasion rate was expressed as a percentage of control. (C, D) AGS cells were treated and then subjected to western blotting to analyze the protein levels of MMP-2 and MMP-9. Values represent the means ± SD of three independent experiments performed in triplicate. *<i>p</i><0.05 and **<i>p</i><0.01 compared with the control group.</p