Normal immune system development and effector function of CD4<sup>+</sup> T cells in <i>Epas1</i><sup>+/−</sup> mice.
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Abstract
<p>(A) Representative flow cytometric analysis of CD4<sup>+</sup> and CD8<sup>+</sup> T-cell populations in the lymph nodes of WT and <i>Epas1</i><sup>+/−</sup> DBA/1J mice. (B) Populations of T<sub>H</sub>1, T<sub>H</sub>2, and T<sub>H</sub>17 cells differentiated from uncommitted CD4<sup>+</sup> T cells of WT and <i>Epas1</i><sup>+/−</sup> DBA/1J mice. (C) IL17A-producing cells identified by flow cytometry (left), and levels of secreted IL17A determined by ELISA (right), from lymphocytes (LN) and splenocytes (SP) of WT and <i>Epas1</i><sup>+/−</sup> DBA/1J mice (<i>n</i> = 8 mice per group) under CIA conditions. (D) mRNA levels of the indicated cytokines in total knee synovial cells isolated from <i>Epas1</i><sup>+/−</sup> DBA/1J mice under CIA conditions or in Ad-<i>Epas1</i>–injected mice (<i>n</i> = 10). The NI condition and Ad-C injection were used as controls. Values are means ± SEM (*<i>p</i><0.01, **<i>p</i><0.005, ***<i>p</i><0.0005).</p
