HIF-2α up-regulates the expression of cytokines, chemokines, and catabolic enzymes in FLS.

Abstract

<p>(A) qRT-PCR analysis (<i>n</i>≥8) of catabolic enzymes and chemokines in FLS infected with Ad-C (800 MOI) or with Ad-<i>Epas1</i> at the indicated MOI for 24 h. (B) qRT-PCR analysis (<i>n</i>≥8) of mRNA levels of HIF-2α, IL6, and TNFα (Left), and ELISA of secreted IL6 and TNFα proteins (Right) in FLS infected with Ad-C (800 MOI) or with Ad-<i>Epas1</i> at the indicated MOI for 24 h. (C) FLS were infected with Ad-C, Ad-<i>Epsa1</i>, or Ad-<i>Hif1a</i> (800 MOI) for 24 h. The indicated proteins were detected by Western blotting (<i>n</i> = 5). Values are means ± SEM (*<i>p</i><0.01, **<i>p</i><0.001).</p