<p>The extracted proteins from transgenic tobacco leaves (A) or seeds (B) were separated by 10% SDS-PAGE, blotted and detected with anti-c-myc monoclonal antibody followed by horseradish peroxidase-linked sheep anti-mouse IgG as a secondary antibody. “+”: anti-hTNFα-VHH-ELP was used as a Western blot standard <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099347#pone.0099347-Conrad1" target="_blank">[19]</a>; Wt: wild type; TSP: total soluble protein. The numbers refer to independent primary transgenic plants.</p
