<p>(<b>A</b>) Section of Chromosome 12 of <i>C. reinhardtii</i> containing the <i>PDS1</i> gene (Cre12.g509650). Exons are shown as boxes; white boxes represent coding regions and black boxes UTRs. Base one is the adenine in the ATG start codon. At base 476 in exon 2, the WT thymine is replaced by guanine in the <i>pds1-nfr1d</i> allele resulting in a phenylalanine to valine missense mutation. Primers used to amplify the gene and an inserted <i>Cla I</i> site are shown. (<b>B</b>) The F131V substitution (red) is found within a highly conserved region of the PDS protein. Coordinates correspond to the <i>C. reinhardtii</i> PDS sequence, where amino acid one is the initiator methionine at the start of the transit peptide.</p
