<p>(<b>A</b>) pNFR1 and pWTPDS1 transformation vectors. The two vectors are distinguished by a single base substitution conferring norflurazon resistance in pNFR1. Shown are coding regions (black boxes), pBluescript vector backbone (thin grey line), extent of cDNA probe used for DNA hybridization and restriction enzyme sites. (<b>B</b>) Map of the <i>PDS1</i> gene (white box) located on chromosome 12 (Chr12). Restriction sites and overlap region of the cDNA probe are shown. (<b>C</b>) Blots of digested DNA from pNFR1 transformants and untransformed cw92 strain hybridized with a <i>PDS1</i> cDNA probe. Restriction enzyme used, band sizes and MW standards are indicated. (<b>D</b>) Protein blot of fractionated total cell protein from pNFR1 cw92 transformants, the cc621 WT strain and the non-transformed cw92 strain incubated with an affinity-purified polyclonal antibody raised against a PDS peptide. The PDS band is arrowed.</p
