<i>In vitro</i> colony-forming unit (CFU) assay.
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Abstract
<p>Data represent mean ± s.d. of colony numbers per plated cells as indicated of 8 male, 8 week-old <i>Tc1</i><sup>−/−</sup> mice, and 8 sex- and age-matched control mice over 3 independent experiments. (A) CFU-granulocyte-macrophage (CFU-GM). (B) CFU-granulocyte-erythroid-monocyte-megakaryocyte (CFU-GEMM). (C) CFU-erythroid (CFU-E). (D) burst-forming unit-erythroid (BFU-E). ***<i>P</i><0.001. (E) Representative flow cytometric analysis of recovered cells from the CFU plates of <i>Tc1<sup>−/−</sup></i> mice and matched control mice.</p