Expression profile comparisons of the five selected miRNAs between 31 distant metastases patients and 27 disease-free patients in the training set by the Mann–Whitney U test.
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Abstract
<p>CT: the threshold cycle to detect fluorescence; ΔCT = CT<sub>miRNA</sub>−CT<sub>U6</sub>, ΔΔCT = ΔCT<sub>poor prognosis</sub>−ΔCT<sub>good prognosis</sub>.</p><p>Patients who developed distant metastasis as first event within 5 years after removal of the primary tumor were considered to have poor prognosis, whereas patients who remained disease free for a minimum of 5 years were defined as having good prognosis. Fold change represented the miRNA expression level of poor prognosis group versus good prognosis group, and was calculated using the equation RQ = 2<sup>−ΔΔCT</sup><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100664#pone.0100664-Livak1" target="_blank">[28]</a>.</p
