Effects of miR-204 on SOX4 expression by luciferase reporter assay.

Abstract

<p>The potential miR-204 binding site at the 3′-UTR of SOX4 mRNA was computationally predicted by Targetscan. Cells were co-transfected with miR-204 mimics (or negative control) with pGL3-SOX4 (or pGL3-SOX4-mut) vector. Luciferase activity was normalized by the ratio of firefly and Renilla luciferase signals. * indicates P<0.05.</p