Enhanced expression of proinflammatory ligands results from neurotoxicity-induced damage: evidence from neuroprotective agents.

Abstract

<p>Mice were administered MPTP, METH, or KA alone or after pretreatment with nomifensine (for MPTP), ethanol (for METH) or diazepam (for KA). Mice were killed at 12 hours post dosing. Total RNA was prepared from striatum (MPTP and METH) or hippocampus (KA) for qRT-PCR analysis of <i>Tnf-α</i>, <i>Osm</i>, <i>Ccl2</i> or <i>Lif</i>. All data points represent mean ± SEM, N = 5. * denotes statistical significance of at least p<0.05 for the neurotoxicant alone or pretreated groups compared to saline controls and # denotes statistical significance of at least p<0.05 for neuroprotectant pretreated groups compared to neurotoxicant only exposed groups. Statistical significance was measured by two-way ANOVA with Fisher's LSD Method of post hoc analysis. See Methods for dosing regimen (single dose METH). Data for <i>Osm</i> and <i>Lif</i> in the MPTP groups was taken from Fig. 8 in Sriram et al., (2004) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102003#pone.0102003-Sriram1" target="_blank">[12]</a>.</p