Immunophenotype analysis of P0-P8 hUC-MSCs by FACS.

Abstract

<p>Cells were from P0-P8 hUC-MSCs and stained with CD29, CD105, CD34, CD45, and CD31 antibodies. The upper half of the figure is the flow histogram of single antibody staining. The shaded area shows the profile of the negative control. The lower half of the figure is CD29-FITC and CD105-PE expression of P0-P8 hUC-MSCs. (A) hUC-MSCs exhibit positive surface expression of integrin marker (CD29), (B) MSC marker (CD105), (C-E) but are negative for hematopoietic lineage markers (CD34, CD45) nor the platelet/endothelial cell adhesion molecule (CD31). The CD29+CD105+ hUC-MSC population accounts for 85%∼99.9% of all cells.</p

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