Effects of native β-D-glucan and three β-D-glucan phosphates, GP-2, GP-4, and GP-5, on cell proliferation (A) and neutral red uptake (B) of RAW264.7 cells. RAW264.7 cells were treated with NG or GP (50, 100, and 500 µg/mL) or LPS (10, 100, and 1000 ng/mL) in different concentrations as described in the Materials and Methods.
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Abstract
<p>After incubation, the viability of RAW264.7 cells was measured by an MTT assay, and the A<sub>570</sub> value was recorded, whereas the amount of neutral red uptake was detected by the A<sub>540</sub> value. The data represent the means ± SD. *<i>p</i><0.05, **<i>p</i><0.01 compared with control. Each point represents the average of three independent experiments.</p
