Characterization of the activity of MCG-TatA.

Abstract

<p>A) Blue-native PAGE of wt-TatA, MCG-TatA and d-MCG-TatA. The mutant proteins retain the characteristic ladder pattern as the wild-type TatA. The molecular masses (kDa) of marker proteins are given on the left. B) Tenfold serial dilution of <i>ΔtatA</i> mutant strain expressing TatA variants on SDS-containing medium. The plates were anaerobically incubated at 35°C for 72 hours and then photographed (see more details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103157#s2" target="_blank">Materials and Methods</a>). The upmost panel corresponds to a positive control experiment using the <i>ΔtatE</i> mutant strain transformed with empty pET-21a plasmids. The lower panel corresponds to the <i>ΔtatA</i> mutant strain transformed with either empty pET-21a plasmids or plasmids carrying wt-TatA gene or mutated variants.</p

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