The effects of pH (A–C), temperature (D) and DTT (E) on chromium-induced plasmid DNA damadge.

Abstract

<p>(A) Plasmid DNA samples were treated with increasing concentrations of CrO₃ (0 µM, 20 µM, 40 µM, 80 µM, and 120 µM) in Tris-HCl buffers of pH 5, pH 7, and pH 10.58 and analyzed with agarose gel electrophoresis. (B) Plasmid DNA samples were treated with increasing concentrations of CrCl₃ (0 µM, 20 µM, 40 µM, 80 µM, and 120 µM) in Tris-HCl buffers of pH 5, pH 7, and pH 10.58 and analyzed with agarose gel electrophoresis. (C) Plasmid DNA samples were treated with or without CrO₃ (80 µM) or CrCl₃ (80 µM) in PBS buffers of pH 4, pH 5, pH 7, and pH 10. (D) Plasmid DNA samples were treated with or without CrO₃ (80 µM) or CrCl₃ (80 µM) at 0°C, 20°C, 30°C, and 37°C. (E) Plasmid DNA samples were treated with or without CrO₃ (80 µM) or CrCl₃ (80 µM) in the presence or absence of increasing concentrations of DTT (0.1 mM, 0.5 mM, 1.5 mM, 5.0 mM and 10 mM). DTT concentration used in the absence of a chromium compound was 10 mM.</p