Construction and expression of NP-NES3 consensus sequence mutants.

Abstract

<p>(A) NP-NES harboring mutations in individual or all Φ residues (leucine was replaced by alanine by site-directed mutagenesis). (B) Expression of wild-type NP and NP-NES3 mutants (Φ1, Φ2, Φ3, and Φ4) was compared by Western blotting with an anti-WSN Ab and an anti-actin MAb.</p