RAxML tree inferred from the alignment of 12 representative V4 sequences of leptocylindracean OTUs from the BioMarKs data, 46 leptocylindracean sequences from GenBank, and 134 reference sequences of bolidomonads, Leptocylindraceae and other diatoms, utilizing the GTRGAMMA base substitution model and Hill Climbing algorithm.
<p><i>Bolidomonas pacifica</i> and <i>B. mediterranea</i> were designated as outgroups. All non-leptocylindracean reference sequences were pruned from the tree following tree construction (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103810#pone.0103810.s001" target="_blank">Figure S1</a> for tree with outgroups included). Bootstrap values were inferred from 100 distinct alternative runs and values <50 are deleted. Each OTU is labelled as follows: the first letter denotes the first letter of the genus, the second letter, the first one of the species; the number denotes the cluster number (numbering starts from zero); the number after the underscore denotes the abundance of the OTU.</p
