Assay of extracellular XlnA and AmlC activity and protein amounts.

Abstract

<p>(A) Detecting XlnA protein secretion by SDS-PAGE and Western blot. WB: Western blot; CB: Commassie Blue Staining. ZJUZ23-26 strains were cultivated for 96 h. (B) Assay of extracellular XlnA activity. ZJUZ23-26 strains were cultivated for 72 h and 96 h. *: p<0.05, **: p>0.05, Chi-squared test. (C) Relative amylase activity from ZJUZ27, ZJUZ28, ZJUZ29 and ZJUZ30. The diameters of the transparent zones around colonies were measured to determine the relative activity.</p

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