VIB1 is not required for cellobiose sensing or signaling.

Abstract

<p>(A) Expression levels of two cellulase genes (<i>cbh-1</i> and <i>gh5-1</i>) were assessed in the Δ3βG; <i>Δvib-1</i> strain versus WT, and the Δ3βG and <i>Δvib-1</i> strains after a shift from sucrose VMM to 0.2% cellobiose versus 2% Avicel. Gene expression levels were measured by relative quantitative-PCR using actin as a control and normalized to expression level when cultures were shifted to VMM with no carbon source. (B) CMCase activity after 24 hrs of growth on 2% cellobiose of the Δ3βG; <i>Δvib-1</i> strain relative to the WT, and the Δ3βG and <i>Δvib-1</i> strains. Measured enzyme activity in arbitrary unit (AU) was normalized against to the mycelial biomass of each culture.</p

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