Regulatory function of CD4⁺CD25^high T cells during remission and clinical flare.

Abstract

<p>PBMCs from healthy donors and from patients with ulcerative colitis were stained for CD4 and CD25 expression. CD4⁺CD25⁻ and CD4⁺CD25^hgh regulatory T cells were sorted by flow cytometry. (A) Gating strategy and purity of sorted CD4⁺CD25⁻ and CD4⁺CD25^high cells are demonstrated as dot plot. (B) CD4⁺CD25⁻ responder T cells were co-cultured at a ratio of 2 to 1 with or without autologous CD4⁺CD25^hgh regulatory T cells under stimulation with Treg suppression inspector beads. The proliferation of responder cells was measured by the loss of CFSE dye, and inhibition was calculated.</p