<p>Cell lysates (120 µg) were separated on 13-cm (isoelectric point [pI] 4–7) linear gradient IPG strips using 12.5% SDS-PAGE. Differentially expressed protein spots are indicated with green squares. (A) Representative 2-DE gels of influenza- and mock-infected MDCK cells. T1/C: PR8-wt infected/mock infected, T3/C: rH1N1NA infected/mock infected, T4/C: rH9N2NA infected/Mock infected, T5/C: rH5N1NA infected/mock infected, T3/T1: rH1N1NA infected/PR8-wt infected, T4/T1: rH9N2NA infected/PR8-wt infected, T5/T1: rH5N1NA infected/PR8-wt infected. (B) Numbers of differentially expressed protein spots detected by 2-DE in virus-infected MDCK cells compared with mock-infected MDCK cells. The number of spots ≥0 indicated the proteins were upregulated, and the number <0 indicated the proteins were downregulated. (C) Numbers of differentially expressed protein spots detected by 2-DE in recombinant viruses compared with wild-type virus (wt-PR8)-infected MDCK cells.</p
