RT-qPCR validation of parts of differentially expressed genes identified by Illumina sequencing.

Abstract

<p>Q1, metacaspase-1-like gene; Q2, ribonuclease 3-like gene; Q3, pathogenesis-related protein (PR-10) gene; Q4, sucrose transporter (SUT1) gene; Q5, vacuolar amino acid transporter 1-like gene; Q6, heat shock protein-like gene. Y, Yacheng05-179; R, “ROC”22; 24 h, 48 h and 120 h, sugarcane buds inoculation with <i>Sporisorium scitamineum</i> at 24 h, 48 h and 120 h, respectively; qPCR, detection results of real-time fluorescent quantitative PCR; log₂FC, fold change of the differential expression genes in the transcriptome.</p