<p>OVA-sensitized BALB/c mice (5% aerosolized-OVA, day21 to 25) were challenged intranasally with PBS (control), rAg85B (rAg85B + isotype control), rAg85B plus neutralizing IL-17 (rAg85B + IL-17Ab) or IL-22 (rAg85B + IL-22Ab), or a combination of both antibodies (rAg85B +IL-17/22Ab) on days 21, 23, and 25. The isotype-matched control antibody was treated with the same time course as neutralization Ab i.n. administration. Lungs from naïve or OVA sensitized BALB/c mice treated with PBS or rAg85B with/without neutralization Ab were sampled one day after the last challenge for histological analysis and quantification of mRNA levels. Lung sections were stained with hematoxylin and eosin (left row, scale bar, 100 mm), Masson's trichrome (center row, scale bar, 100 mm), α-smooth muscle actin (right row, scale bar, 50 mm). Numbers in quadrants indicate the score scale from 0 to 5 in each (<i>A</i>). Real-time RT-PCR was performed for the indicated molecules expression on RNA isolated from individual mice lungs (<i>B</i>). Data are representative of at least two independent experiments (*P<0.05, **P<0.01 compared with rAg85B+isotype control challenged group. error bars, s.d.; n = 6 mice).</p
