qRT-PCR of 8 transcripts for validating the DGE results.
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Abstract
<p>The horizontal axis identifies the 8 transcripts examined by qRT-PCR; the vertical axis shows the relative gene expression level in deformed (individuals 1 and 2) versus normal (individuals 3, 4, 5, and 6) beak tissues. The 4 pairs of individuals were all full sibs. The first bar shows the value obtained from DEG using 1 and 6. <i>NPM3</i> = nucleophosmin/nucleoplasmin 3; <i>LPL</i> = lipoprotein lipase; <i>BMP4</i> = bone morphogenetic protein 4; <i>SGOL1</i> = shugoshin-like 1; <i>KRT19</i> = Keratin 19; <i>sKer</i> = similar to Scale keratin; <i>MMP7</i> = matrix metalloproteinase 7; <i>MUC</i> = mucin protein.</p