<p>A. Primary culture of fibroblasts from biopsy of tail-tip from C57BL/6 mouse. Scale bar = 200 µm. B, C. Morphology of C57BL/6 iPS colonies cultured in control ES medium (B) and SC1-supplemented medium (C), respectively. Scale bar = 50 µm. D. Alkaline phosphatase activity of C57BL/6 iPS cells. Arrows indicate the weakly stained colonies. Scale bar = 25 µm. E. RT-PCR detection of ES specific and fibroblast markers from C57BL/6 tail-tip fibroblast and iPS cells. F, G. Immunocytochemistry of C57BL/6 iPS cells by Oct4 (green) and Nanog (red; F) and Sox2 (green) and SSEA-1 (red; G) markers, DNA were counterstained by TO-PRO-3 (blue) Scale bar = 25 µm.</p
