Anti-rBdRAP-1 antibodies identify a specific ∼46 kDa protein in parasite lysate.

Abstract

<p>A, Immunoblotting of proteins from in vitro cultured <i>B. divergens</i> lysate showed anti-rBdRAP-1 antibodies (lane 1) are able to detect native BdRAP-1, as shown by the presence of a single band at ∼46 kDa. Pre-immune rabbit serum (lane 2) did not react with any native antigens. B, Immunoprecipitation with lysate from [³⁵S] methionine/cysteine-labelled <i>B. divergens</i> cultures showed native BdRAP-1 is present in the culture pellet (lane 1), as observed by the presence of a single band at ∼46 kDa. BdRAP-1 is secreted into the culture supernatant as a processed product, as shown by the presence of an abundant ∼17 kDa product, and a much less abundant product of ∼34 kDa, in the culture supernatant (lane 2; indicated by arrows).</p