Platelet depletion did not affect proliferation of capillary-associated cells or myocytes.

Abstract

<p>A) The total number of proliferating cells increased slightly in tissue after induction of capillary sprouting, but was not affected by platelet depletion. B) Capillary-associated cell proliferation was unaffected by platelet depletion. C) The increase in proliferating cell number with sprouting results from increased interstitial cell proliferation. D) The absence of platelets did not impair the proliferation of myocytes following muscle overload. E) BrdU labelling was performed in conjunction with rhodamine <i>Griffonia simplicifolia</i> lectin-1 staining (bottom left panel) for visualisation of vasculature, and DAPI (top right panel) to ensure BrdU staining observed was localised to nuclei. A merge (bottom right panel) of the three other panels with three BrdU-labelled interstitial cells (white arrows) is shown. Imaging was performed with fluorescent light microscopy as described in the methods at ×40 magnification. *<i>P</i><0.05 <i>vs.</i> contralateral.</p