Genotyping of MHC class II gene regions by PCR-RFLP.

Abstract

<p>A 279-bp fragment of MHC IIB exon 2 was amplified from five founder genomes (A–E). PCR products were digested with <i>Pst</i>I, <i>Rsa</i>I, or <i>Sal</i>I and digested fragments were analyzed by 3% agarose gel. Non-digested fragments with <i>Rsa</i>I, <i>Pst</i>I-digested fragments, and <i>Sal</i>I-digested fragments represented type II (<i>DAB1*01</i> in HP1), type IV (<i>DAB1*02</i> in HP2), and type III (<i>DAB1*03</i> in HP3), respectively (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108506#pone-0108506-t001" target="_blank">Table 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108506#pone-0108506-g001" target="_blank">Figure 1</a>).</p