Detection of MHC class II gene regions by Southern blotting.

Abstract

<p>Genomic DNA from five founders (A–E) was digested with <i>Bam</i>HI and <i>Eco</i>RI and hybridized with a mixture of three probes (MHC IIA exon 3, MHC IIB exon 2, and MHC IIB exon 3). Three bands of approximately 8, 13, and 18 kb represented HP1, HP2, and HP3, respectively.</p