Loss of Cep120 results in failed expansion of granule neuron progenitors (GNPs), due to lack of a response to Hedgehog signaling.

Abstract

<p>(A–F) Sagittal sections of P1, P7, and P14 wild type and mutant cerebellums were coimmunostained for Pax6 (red), Calb1 (green), and nuclei (DAPI, blue). Pax6 and Calb1 label GNPs and Purkinje cells, respectively. Framed areas in panels A–F are enlarged in A′–F′. EGL, external granule cell layer; PCL, Purkinje cell layer; ML, molecular layer; IGL, inner granule layer. Genotypes are shown to the left. (G–H) LacZ staining of the P7 cerebellum is positive in wild type animals (G), but negative in the Cep120 mutant (H). Genotypes are indicated on both sides. Cerebellums are outlined, and cerebellar posterior lobes are indicated by asterisks.</p