Failed centriole duplication, maturation, and ciliogenesis in cerebellar granule neuron progenitors (CGNPs) and ependymal cells in the Cep120 mutant.

Abstract

<p>(A–D) Sagittal brain sections of P14 mice with the indicated genotypes were coimmunostained for acetylated α ˜tubulin and Arl13b, cilia markers. The fourth ventricular choroid plexus is circled. Framed areas in panels A and B are enlarged in A′/A″ and B′/B″. Panels A″ and B″ show representative areas of ependyma near the fourth ventricle. Panels C and D show representative areas of CGNPs. Arrowheads indicate representative cilia. Note that cilia develop in the <i>Cep120^f/-</i>; <i>nes-Cre</i> choroid plexus, but not in ependymal cells and CGNPs. (G–L) Sagittal cerebellar sections of P14 mice with the indicated genotypes were coimmunostained for γ ˜tubulin and Cep120, Odf2, or Ta3, as shown. Note that very few centrioles are present in <i>Cep120^f/-</i>; <i>nes-Cre</i> CGNPs, relative to wild type CGNPs. Arrowheads indicate one or two representative centrioles in each panel.</p